In a context of increasing use and transboundary shipment of sterile insects, this updated FAO/IAEA guideline provides a compilation of the standardized processes currently used in most of the fruit fly Sterile Insect Technique (SIT) applications world-wide. It presents harmonized and optimized technologies for all post-mass-rearing processes related to the application of SIT, from pupal shipment to fly emergence through to insect release. It includes updates on methods to estimate sterile to fertile fly ratios and densities, novel packing, holding and release equipment and procedures, and a much expanded chapter on the use of Global Information Systems (GIS).