This publication provides guidance on the most appropriate methods for generating data on Vibrio spp., a foodborne pathogen that affects seafood. One of the challenges in undertaking global risk assessments for halophilic Vibrio spp. has been identifying datasets that are comparable and representative of the range of conditions in which halophilic Vibrio spp. grow and become a problem for seafood safety. A range of potential data collection methods are presented, from culture-based to molecular, and the publication proposes means of selecting the most appropriate method for specific end uses, for example harvest area monitoring, post-harvest process verification, end product monitoring, or outbreak investigation. Data requirements that could support national and regional risk assessments are also addressed. This volume and others in this series will be useful for seafood safety risk assessors and risk managers, governments and regulatory agencies, seafood producers and processers, laboratories and others with an interest in Vibrio spp. in seafood.